Separation of Alkaloids by Paper Chromatography

Separation of Plant Pigments by Paper Chromatography

Anyone who has ever played on grass or had an unplanned encounter with a bush knows that plants contain pigments which adhere to varying degrees to fabric (and skin!). The fact that a small industry has risen up in aid of attempts to remove stains like these and many others shows that not all of the molecules can be treated in the same way if their removal is desired. Part of the reason behind ...

Separation of Amino Acids by Paper Chromatography

Chromatography is a common technique for separating chemical substances. The prefix “chroma,” which suggests “color,” comes from the fact that some of the earliest applications of chromatography were to separate components of the green pigment, chlorophyll. You may have already used this method to separate the colored components in ink. In this experiment you will use chromatography to separate...

Paper Chromatography: Separation and

The example of column chromatography (Figure 1) demonstrates the typical features found in this analytical technique. The diagram shows an experiment where a two-component mixture is subjected to column chromatography. The column is packed with a solid material called the stationary phase. A liquid solvent or eluting solution is poured into the column and completely wets the solid packing mater...

Note on the identification of alkaloids by paper chromatography.

Ix 1949, Munier and Macheboeuf (1) suggested the use of ascending paper chromatography for the isolation and identification of various alkaloids and various organic nitrogen compounds. Their procedure was most successfully applied by Mannering and his associates (2) to the detection of opium alkaloids in urine and tissues. The conditions set. by the latter workers led to such interesting result...

Study of phenylketonuria by paper chromatography